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Datasheet Protocol


1 Kit
31.2 pg/ml - 2000 pg/ml
$589.00 /Per Item
Availability: Ships in approximately 21 days
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Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 419 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

Product Details

Mouse TPO AccuSignal ELISA Kit - KOA0310
C mpl ligand, Megakaryocyte colony stimulating factor, Megakaryocyte growth and development factor, Megakaryocyte stimulating factor, MGC164194, MGDF, MKCSF, ML, MPL ligand, MPLLG, Myeloproliferative leukemia virus oncogene ligand, OTTHUMP00000210504, THPO, TPO
31.2 pg/ml - 2000 pg/ml

Target Details

TPO  - View All TPO Products
Expression system for standard: NSO; Immunogen sequence: S22-T356
Natural and recombinant mouse TPO. There is no detectable cross-reactivity with other relevant proteins.

Application Details

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse TPO standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse ell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TPO standard solution and each sample be measured in duplicate.


Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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