Mouse DAN - NBL1 ELISA Kit
Mouse DAN/NBL1 Accusignal ELISA Kit
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Mouse DAN - NBL1 ELISA Kit

1 Kit
62.5 pg/ml - 4000 pg/ml
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Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Application Note

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse DAN standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse DAN standard solution and each sample is measured in duplicate.


Natural and recombinant mouse DAN. There is no detectable cross-reactivity with other relevant proteins.

Mouse DAN/NBL1 AccuSignal ELISA Kit - KOA0439
D1S1733E, DAN, DAN domain family member 1, DAND 1, DAND1, Differential screening selected gene aberrant in neuroblastoma, MGC8972, NB, NBL 1, Neuroblastoma candidate region suppression of tumorigenicity 1, Neuroblastoma suppression of tumorigenicity 1, Neuroblastoma suppressor of tumorigenicity 1, NO 3, NO3, Zinc finger protein DAN
Expression system for standard: NSO; Immunogen sequence: A17-D178
62.5 pg/ml - 4000 pg/ml
<10 pg/ml
Heparin Sodium
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.