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Datasheet Protocol

Human Tenascin-C - TNC ELISA Kit

KOA0673
1 Kit
ELISA
Human
93.7 pg/ml - 6000 pg/ml
$589.00 /Per Item
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Product Details

Human Tenascin-C/TNC AccuSignal ELISA Kit - KOA0673
150 225, Cytotactin, Glioma associated extracellular matrix antigen, Glioma-associated-extracellular matrix antigen, GMEM, GP 150 225, GP 150-225 GP, Hexabrachion, HXB, JI, MGC167029, Miotendinous antigen, Myotendinous antigen, Neuronectin, TENA_HUMAN, Tenascin, Tenascin-C, Tenascin-C isoform 14/AD1/16, TenascinC, TN, TN C, TN-C, TNC
ELISA Kit
93.7 pg/ml - 6000 pg/ml

Target Details

TNC  - View All TNC Products
Human
Expression system for standard: NSO; Immunogen sequence: G23-P625
Natural and recombinant human Tenascin-C. There is no detectable cross-reactivity with other relevant proteins.
P24821 - UniProtKB
NP_002151.2 - NCBI Protein

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 419pg/ml, 187.5pg/ml, 93.7pg/m human Tenascin-C standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human Tenascin-C standard solution and each sample be measured in duplicate.

Formulation

Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

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