Human Syndecan-4 - SDC4 ELISA Kit
KOA0819
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human Syndecan-4/SDC4 AccuSignal ELISA Kit - KOA0819
Amphiglycan, MGC22217, OTTHUMP00000031788, ryudocan amphiglycan, Ryudocan, Ryudocan core protein, SDC 4, SDC4, SDC4_HUMAN, SYND 4, SYND4, syndecan 4, syndecan 4 (amphiglycan, ryudocan), syndecan proteoglycan 4, Syndecan-4, Syndecan4
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
SDC4 - View All SDC4 Products
Human
Expression system for standard: NSO; Immunogen sequence: E19-E145
Natural and recombinant human Syndecan-4. There is no detectable cross-reactivity with other relevant proteins.
P31431 - UniProtKB
NP_002990.2 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Syndecan-4 standard solution and each sample be measured in duplicate.
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
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