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Datasheet Protocol


1 Kit
7.8 pg/ml - 500 pg/ml
$589.00 /Per Item
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Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.

Product Details

Human sTNFsR II AccuSignal ELISA Kit - KOA0322
CD120b, Etanercept, p75, p80 TNF alpha receptor, TBP2, TBPII, TNF R2, TNFBR, TNFR-II, TNFR2, TNFRII, Tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor superfamily member 1b, membrane form, Tumor necrosis factor receptor type II
7.8 pg/ml - 500 pg/ml

Target Details

TNFRSF1B  - View All TNFRSF1B Products
Expression system for standard: NSO; Immunogen sequence: L23-D257
Natural and recombinant human sTNFsRⅡ. There is no detectable cross-reactivity with other relevant proteins.
P20333 - UniProtKB
NP_001057.1 - NCBI Protein

Application Details

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human sTNFsRⅡ standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. We recommend that each human sTNFsRⅡ standard solution and each sample is measured in duplicate.


Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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