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Datasheet Protocol


1 Kit
62.5 pg/ml - 4000 pg/ml
$639.00 /Per Item
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MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer(NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.

Product Details

Human MICB AccuSignal ELISA Kit - KOA0593
MHC class I chain related protein B, MHC class I like molecule PERB11.2 IMX, MHC class I mic B antigen, MHC class I polypeptide related sequence B, MHC class I polypeptide-related sequence B, MIC B, MIC-B, MICB, MICB_HUMAN, PERB11.2, Stress inducible class I homolog
62.5 pg/ml - 4000 pg/ml

Target Details

MICB  - View All MICB Products
Expression system for standard: NSO; Immunogen sequence: A23-G298
Natural and recombinant human MICB. There is no detectable cross-reactivity with other relevant proteins.
Q29980 - UniProtKB
NP_001276089.1 - NCBI Protein

Application Details

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human MICB standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MICB standard solution and each sample be measured in duplicate.


Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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