Human CSF3R - G-CSF R ELISA Kit
Human CSF3R/G-CSF R Accusignal ELISA Kit
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Datasheet

Human CSF3R - G-CSF R ELISA Kit

KOA0452
1 Kit
ELISA
Human
31.2 pg/ml - 2000 pg/ml
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Description

Background

The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.

Application Note

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human CSF3R standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.

Purity/Specificity

Natural and recombinant human CSF3R. There is no detectable cross-reactivity with other relevant proteins.

Human CSF3R/G-CSF R AccuSignal ELISA Kit - KOA0452
CD 114, CD114, CD114 antigen, Colony stimulating factor 3 receptor (granulocyte), Colony stimulating factor 3 receptor, CSF 3R, CSF3R, CSF3R_HUMAN, Csfgr, G CSF R, G-CSF receptor, G-CSF-R, GCSFR, Granulocyte colony stimulating factor receptor, Granulocyte colony-stimulating factor receptor, OTTHUMP00000009703, OTTHUMP00000009704, OTTHUMP00000009705
CSF3R
ELISA
Human
Expression system for standard: E.cNSO; Immunogen sequence: E25-P621
ELISA Kit
31.2 pg/ml - 2000 pg/ml
<10 pg/ml
Heparin Sodium
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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