HRP Western Blot Anti-Mouse IgG Antibody
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Datasheet

HRP Western Blot Anti-Mouse IgG Antibody

Goat Polyclonal

KCB002
100 µg
WB
Goat
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Description

Background

HRP is used in applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal and HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Chemiluminescence is the best all-around method for western blot detection as these systems eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. These methods are also unmatched in the speed and sensitivity compared to traditional alternatives, utilizing film to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. This item is a component of kit (KCA002). The Kit contains all the reagents necessary to perform chemiluminescent Western Blots to detect mouse primary antibodies.

Application Note

Western Blot Analysis

HRP Western Blot Anti-Mouse IgG Antibody - KCB002
Anti-mouse IgG HRP conjugated, HRP-linked Antibody, Anti-Mouse HRP Secondary Antibody, Horseradish Peroxidase-Conjugated Antibody
WB
Goat
Peroxidase (Horseradish)
Polyclonal
Chemiluminescent Western Blot Kit
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
100 µL
Restore with deionized water (or equivalent)
Wet Ice
See kit insert for complete instructions.
See kit insert for complete instructions.
Muena NA et al. Induction of SARS-CoV-2 neutralizing antibodies by CoronaVac and BNT162b2 vaccines in naïve and previously infected individuals. EBioMedicine. (2022)
Applications
IP, Co-IP
Tomicic MT et al. Oxaliplatin-Induced Senescence in Colorectal Cancer Cells Depends on p14 ARF-Mediated Sustained p53 Activation. Cancers (Basel). (2021)
Applications
WB, IB, PCA
Schwarzenbach C et al. Targeting c-IAP1, c-IAP2, and Bcl-2 Eliminates Senescent Glioblastoma Cells Following Temozolomide Treatment. Cancers (Basel). (2021)
Applications
WB, IB, PCA
Gu Z et al. The SUMOylation of TAB2 mediated by TRIM60 inhibits MAPK/NF-κB activation and the innate immune response. Cell Mol Immunol. (2021)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.