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Datasheet

DNA Damage (8-OHdG) ELISA Kit

Monoclonal

KOA0887
1 Kit
Broad
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$552.00 /Per Item
Availability: Ships in approximately 14 days
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/ml as determined by LC-MS.

Synonyms

8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, DNA Damage ELISA Kit, 8OHdG ELISA Kit, 8-ohdg ELISA Kit, DNA Damage Enzyme-Linked Immunosorbent Assay, 8-OHdG ELISA Kit

Application Note

Colorimetric Competitive ELISA (Enzyme-linked Immunosorbent Assay) assay for quantification of 8-hydroxy-2-deoxy Guanosine in Cell lysates, Plasma, Sample Matrices, and Urine. Sensitivity: 0.59 ng/mL. Range: 0.94-60 ng/mL.

Purity/Specificity

This kit contains: 96 wells coated plate, Target Protein Standard, HRP Conjugated Monoclonal Antibody, Diluent, Wash buffer, TMB Substrate and stop reagent, see protocol for additional information.

Storage Condition

See kit insert for component storage. Store kit at 4°C and -20°C.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

DNA Damage AccuSignal ELISA Kit - KOA0887
Broad
Peroxidase (Horseradish)
Monoclonal
ELISA Kit
Wet Ice
Expiration date is one (1) year from date of opening.
1 Kit

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