AKT ISOFORM ANTIBODY SAMPLER KIT
Flow Cytometry of Mouse anti-AKT1 APC
Monoclonal mouse anti AKT1 - WB - Cell lines
Monoclonal mouse anti AKT1 - ELISA
AKT1 Antibody - Western Blot
AKT3 Antibody - Western Blot
AKT2 Antibody - Western Blot
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

AKT ISOFORM ANTIBODY SAMPLER KIT

Mouse Monoclonal various

K401
1 Kit
Human
Mouse
Have a Question? Ask a Specialist
$319.00 /Per Item
Availability: Call for availability
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This primary antibody kit is designed to detect and quantify endogenous protein levels of human AKT1, AKT2 and AKT3. Each antibody is designed to recognize a specific isoform of AKT. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibodies are ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Application Note

Anti-AKT Antibodies are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT proteins by western blotting in the appropriate cell lysate or extract. All 3 primary monoclonal antibodies reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. This sampler kit consists of sample sizes of anti-AKT1, anti-AKT2 and anti-AKT3 mouse monoclonal antibody and HRP conjugated anti-Mouse IgG secondary antibody (p/n 200-301-I51S; 200-301-BR8S; 200-301-J36S and 610-403-C46S, respectively).

Purity/Specificity

This primary antibody kit has been carefully chosen to recognize all 3 isoforms of AKT. Anti-AKT1 antibody is directed against human AKT1. Anti-AKT2 antibody is directed against human AKT2. Anti-AKT3 antibody is directed against human AKT3. The antibodies detect both unphosphorylated and phosphorylated forms of the protein. The antibodies were purified from ascites by Protein A chromatography.

Storage Condition

Each vial contains a relatively low volume of reagent (25 µL). Store vial at -20° C or below prior to opening. To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

AKT ISOFORM ANTIBODY SAMPLER KIT - K401
AKT isoform antibody kit, AKT-1, AKT-2, AKT-3, Protein kinase Akt antibody, Protein kinase B antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, antibody sampler,
AKT1, AKT2, AKT3
Human
Human
Mouse
Monoclonal
various
IgG2a
Peptide
All Anti-AKT isoform specific Antibodies were produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1, AKT2 or AKT3 protein.
Liquid and Lyophilized
Dry Ice
Expiration date is one (1) year from date of opening.
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.