AKT3 Western Chemiluminescent Blotting Kit
Immunofluorescence - Mouse monoclonal anti AKT3
ELISA - Mouse Monoclonal anti-AKT3
AKT3 Antibody - Western Blot
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AKT3 Western Chemiluminescent Blotting Kit

Mouse Monoclonal 9A5.H9.G7 IgG1 kappa

1 Kit
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Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT3 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT3 protein. AKT3 Western Blotting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Application Note

Use Rockland Immunochemicals’ Anti-AKT3 Chemiluminescent Kit for Western Blotting for detection of human AKT3 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT3 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.


The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT1 and AKT2 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

AKT3 Western Blot Kit - KCAE75
AKT 3 western blotting, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, western bloting kit, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit
Chemiluminescent Western Blot Kit
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
Dry Ice
See kit insert for complete instructions.
See kit insert for complete instructions.

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This material is subject to proprietary rights and is sold under license from ROCKLAND IMMUNOCHEMICALS Inc. This product is licensed for sale only for 'research-use' only. There is no implied license hereunder for any commercial use. FemtoMax® is a trademark of ROCKLAND IMMUNOCHEMICALS Inc. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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