Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

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Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Name: Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Brand: Rockland Immunochemicals
Price: 189.0 USD
Availability: InStock

Donkey Polyclonal

Item No. 610-705-002

Size: 1 mg

Physical State: Liquid (sterile filtered)

Applications: ELISA

Reactivity: Mouse

Host Species: Donkey

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Specifications for Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Product Details

Description: Donkey Anti-Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated - 610-705-002

Synonyms: Donkey anti-Mouse IgG alkaline phosphatase conjugated Antibody, Donkey anti-Mouse IgG Antibody alk phos conjugation

Host Species: Donkey

Specificity: IgG (H&L)

Conjugate: Alkaline Phosphatase (AP)

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen: Mouse IgG whole molecule

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Mouse IgG, and Mouse Serum.

Application Details

Tested Applications: ELISA

Application Note: Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Formulation

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Preservative: 0.1% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgG Alkaline Phosphatase Antibody generated in donkey detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Related Protocols

ELISA Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.