NMDA NR2B Subunit Phospho S1166 Antibody

Datasheet

NMDA NR2B Subunit Phospho S1166 Antibody

Name: NMDA NR2B Subunit Phospho S1166 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-X17

Size: 100 µL

Applications: WB

Reactivity: Mouse, Rat

Host Species: Rabbit

$475.00 /Per Item

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Background

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Phosphorylation of Ser1166 is thought to play an essential role in memory and neuronal development. NMDA pS1166 Receptor NR2B Subunit Antibody is ideal for researchers interested in neuroscience research.

Expand Background

Specifications for NMDA NR2B Subunit Phospho S1166 Antibody

Product Details

Description: Anti-NMDA NR2B Subunit pS1166 (RABBIT) Antibody - 600-401-X17

Synonyms: Glutamate receptor ionotropic, NMDA 2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: Grin2b - View All Grin2b Products

Reactivity: Mouse, Rat

PTM Specificity: Phosphorylation

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Phospho-Ser1166 NMDA Receptor NR2B Subunit Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1166 of rat NMDA Receptor NR2B Subunit conjugated to KLH.

Purity/Specificity: Anti-Phospho-Ser1166 NMDA Receptor NR2B Subunit Antibody is directed against NMDA protein phosphorylated at S1166. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated NMDA. Minimal reactivity occurs against non-phosphorylated NMDA. Reactivity against NMDA occurs from rat sources. However, reactivity is also expected against canine, human, non-human primate, and Xenopus based on 100% sequence homology.

Database Links:

UniProtKB - Q00960

GeneID - 24410

NCBI - NP_036706.1

Application Details

Tested Applications: WB

Application Note: Anti-Phospho-Ser1166 NMDA Receptor NR2B Subunit Antibody is tested for Western Blots and is specific for the ~180 kDa NMDAR NR2B subunit phosphorylated at Ser1166. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: Titrated value sufficient to run approximately 10 mini blots.

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Preservative: None

Stabilizer: 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.