Mouse IgG Fc Antibody Peroxidase Conjugated

Datasheet

Mouse IgG Fc Antibody Peroxidase Conjugated

Name: Mouse IgG Fc Antibody Peroxidase Conjugated
Brand: Rockland Immunochemicals
Price: 359.0 USD
Availability: OutOfStock
Rating: 5 (2 reviews)

Goat Polyclonal

2 References

Item No. 210-1303

Size: 20 mg

Physical State: Lyophilized

Applications: WB

Reactivity: Mouse

Host Species: Goat

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Specifications for Mouse IgG Fc Antibody Peroxidase Conjugated

Product Details

Description: Goat Anti-Mouse IgG Fc Antibody Peroxidase Conjugated - 210-1303

Synonyms: Mouse IgG F(c) Antibody, Gt-a-Mouse F(c), Mouse F(c) Antibody in goat, Mouse F(c) Secondary Antibody, goat anti-Mouse IgG F(c) fragment Peroxidase Conjugated Antibody

Host Species: Goat

Specificity: IgG Fc

Conjugate: Peroxidase (HRP)

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen Type: Native Protein

Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.

Purity/Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).

Application Details

Suggested Applications: WB - View References

Application Note: Mouse IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Formulation

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

References (2)

Shimizu T et al. (2013). Interleukin-8 stimulates progesterone production via the MEK pathway in ovarian theca cells. Mol Cell Biochem.

Applications

WB, IB, PCA

PubMed

Murayama C et al. (2012). Luteinizing hormone (LH) regulates production of androstenedione and progesterone via control of histone acetylation of StAR and CYP17 promoters in ovarian theca cells Mol Cell Endocrinol.

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.