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Datasheet

Cul4A Antibody

Rabbit Polyclonal

2 References
100-401-A04
100 µL
WB, IHC, IP
Human
Rabbit
$426.00 /Per Item
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Background

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates.  Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits.  Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Product Details

Anti-Cul4A (N-terminal specific) (RABBIT) Antibody - 100-401-A04
rabbit anti-Cul4A Antibody, 2810470J21Rik antibody, AW495282 antibody, CUL 4A antibody, CUL4A antibody, Cul4a protein antibody, MGC36573 antibody, MGC64071 antibody, CUL, Cullin
Rabbit
Polyclonal
Antiserum

Target Details

CUL4A  - View All CUL4A Products
Human
Conjugated Peptide
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cul4A coupled to KLH.
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 4A.  Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.

Application Details

IHC, WB
IP  - View References
Anti-Cul4A has been tested by western blot, immunoprecipitation, and immunohistochemistry.  The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others).  This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1.  An 87.6 kDa band corresponding to human Cul4A is detected.  Most cell lines expressing Cul4A can be used as a positive control.  Researchers should determine optimal titers for other applications.

Formulation

Liquid (sterile filtered)
85 mg/mL by Refractometry
None
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). FBXW8 regulates G1 and S phases of cell cycle progression by restricting β‐TrCP1 function. FEBS J.
Applications
Undefined
(). Cul4A targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis. Blood.
Applications
IP, Co-IP

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.