Green fluorescent protein (GFP) has revolutionized the field of molecular and cell biology as a valuable tool for tracking protein expression and localization in living cells. GFP is a 27 KDa cytoplasmic protein that was first purified from the jellyfish Aequorea victoria. Consistent with other fluorescent proteins, the fluorophore of GFP is formed by autocatalysis from three consecutive amino acid residues within a polypeptide chain. To maximize the utility of GFP in certain applications such as western blotting, immunoprecipitation, and fluorescent microscopy, researchers require reliable and specific GFP antibodies.

This article will provide a detailed overview of GFP antibodies from chicken, goat, mouse, and rabbit hosts, and how they can address different concerns in scientific research.