Collagen Antibodies

November 08, 2021

What Are Collagens? Understanding the Basics

Collagens are essential components of the extracellular matrix and continue to be important targets for technology and therapy. As the chief structural component of skin, collagen is the most abundant protein in the body, representing approximately 30% of its dry weight. The use of collagen has become increasingly prevalent in various medical applications such as implants, organ replacement, tissue equivalents, arterial plugs, cosmetic surgery, surgical sutures, and surgical dressings for wounds and burns. These advances were predicated on the use of anti-collagen antibodies to allow researchers to detect collagens, including type-specific collagens, in cell biology research. 

Collagen Helical Structure

Illustration depicting the triple helical structure of collagen

 

Variations in collagen structure create functional diversity essential for distinct biological features in the various types of tissues of the body. Based on their supramolecular structures, collagens are divided into two main classes: fibril-forming collagens (type I, II, III, and V) and non-fibril-forming collagens (type IV and VI). In humans, there are more than 20 unique procollagens that are subjected to various post-translational modifications resulting in great diversity between collagen types.

Collagen Antibodies

Precise insights into the behavior and physiology of cells can be made by researchers, especially when using a type-specific collagen antibody to study collagen’s bio-distribution, interactions between associated cells, mechanisms of cell adhesion, and cell development and differentiation. Because of the high specificity of type-specific anti-collagen antibodies, which often differentiate one collagen type from another by subtle differences in the triple-helical structure of native collagen molecules, it is important to select the correct antibody that is “fit-for-purpose” to detect a specific type of collagen in the right tissue and immunoassay. Rocklands collagen antibodies have been cited in more than 300 research publications, establishing them as the preferred option in nearly every circumstance when it comes to collagen research.

 

Collagen Antibodies

Product Clonality Reactivity Applications
Collagen Type I Antibody Polyclonal Human, Mouse, Rat, Bovine, Pig WB, IHC, IF, FC, IP, Multiplex, ELISA
Collagen Type II Antibody Polyclonal Human, Bovine IHC, IF
Collagen Type III Antibody Polyclonal Human, Bovine, Pig WB, IHC, IF, FC, ELISA
Collagen Type IV Antibody Polyclonal Human, Bovine WB, IHC, IF, Multiplex
Collagen Type V Antibody Polyclonal Human, Bovine IHC, IF, Multiplex
Collagen Type VI Antibody Polyclonal Human, Bovine IHC, IF

 

Collagen Antibody Controls

It cannot be understated that the appropriate controls should be included in experimental design when type-specific anti-collagen antibodies are used to ensure the success of the experiment and data reproducibility. Only highly-purified and type-specific collagen proteins should be used as controls that are themselves free of impurities, including serum proteins, other collagen proteins, and non-collagen extracellular matrix proteins. Standards must not only be physically pure for collagen but also be immunologically pure for the type of collagen to be used as controls in experiments of this type. Type-specific anti-collagen antibodies should be selected that are appropriate for the detection of collagens present in the tissue to be tested, and these antibodies should be used with protocols designed to optimize antibody performance for the collection of high-quality data ultimately intended to help better understand the biology of these proteins.

 

Human Collagen Proteins

Product Origin Applications
Human Collagen Type I Human SDS-PAGE, WB, Cellular Assay
Human Collagen Type II Human SDS-PAGE, WB
Human Collagen Type III Human SDS-PAGE, WB
Human Collagen Type IV Human SDS-PAGE, ELISA
Human Collagen Type V Human SDS-PAGE
Human Collagen Type VI Human SDS-PAGE, Cellular Assay

 

Bovine Collagen Proteins

Product Origin Applications
Bovine Collagen Type I Bovine SDS-PAGE, WB
Bovine Collagen Type II Bovine SDS-PAGE
Bovine Collagen Type III Bovine SDS-PAGE
Bovine Collagen Type IV Bovine SDS-PAGE
Bovine Collagen Type V Bovine SDS-PAGE
Bovine Collagen Type VI Bovine  

 

Top Cited Collagen Antibodies

600-401-103-0.1
Collagen Type I Antibody
226 References
Applications
WB, ELISA, IHC, IF, FC, Dot Blot, IP, Multiplex
600-401-104-0.1
Collagen Type II Antibody
26 References
Applications
IHC, IF, Dot Blot, Multiplex
600-401-105-0.1
Collagen Type III Antibody
47 References
Applications
WB, ELISA, IHC, IF, FC, Dot Blot, Multiplex
600-401-106-0.1
Collagen Type IV Antibody
41 References
Applications
WB, IHC, IF, Dot Blot, Multiplex

Frequently Asked Questions

The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).
The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.
The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.
Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.
The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.