Analysis of Dialyzed Flask Technology for Antibody Production

Efficient and cost effective hybridoma culture is essential to small and large scale monoclonal antibody production for research purposes. This study evaluates multiple aspects of two existing production methods and a new dialyzed cell culture fl ask method for culturing hybridoma cells. The flask separates the cell cultivation compartment from the cell culture media via a 10kDa cut off limit membrane. This method allows for multiple harvests, longer run times and a super-concentrated supernatant.