Efficient and cost effective hybridoma culture is essential to small and large scale monoclonal antibody production for research purposes. This study evaluates multiple aspects of two existing production methods and a new dialyzed cell culture fl ask method for culturing hybridoma cells. The flask separates the cell cultivation compartment from the cell culture media via a 10kDa cut off limit membrane. This method allows for multiple harvests, longer run times and a super-concentrated supernatant.