TMB ELISA Peroxidase Substrate
TMBE ELISA Peroxidase Substrate - ELISA
TMBE ELISA Peroxidase Substrate - Buffers
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Datasheet

TMB ELISA Peroxidase Substrate

TMBE-100 TMBE-1000 TMBE-8000 TMBE-20L
100 mL 1 L 8 L 20 L
ELISA
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$106.00 /Per Item
$239.00 /Per Item
$1,433.00 /Per Item
$3,582.00 /Per Item
100 mL $106.00
1 L $239.00
8 L $1,433.00
20 L $3,582.00
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Description

Background

TMB ELISA Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow. TMB ELISA Peroxidase Substrate is ideal for investigators in Microbiology, Immunology, and Cancer research.

Application Note

TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.

Purity/Specificity

pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Storage Condition

Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.

TMB ELISA PEROXIDASE SUBSTRATE - TMBE-100
3,3’,5,5’-Tetramethylbenzidine, TMB ELISA substrate, TMBE Substrate, chromogenic ELISA substrate
ELISA
Liquid - clear to very light blue colored liquid
Ambient
Expiration date is one (1) year from date of opening.
1x
Proprietary buffer with enhancer
Quach HQ et al. Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA. (2022)
Applications
EIA, ELISA
Kratzer B et al. Immunological imprint of COVID-19 on human peripheral blood leukocyte populations. (2021)
Applications
E, EIA
Pandit M et al. LKB1-PTEN axis controls Th1 and Th17 cell differentiation via regulating mTORC1. (2021)
Applications
Undefined
Kukla DA. et. al. Microscale Collagen and Fibroblast Interactions Enhance Primary Human Hepatocyte Functions in Three-Dimensional Models. (2020)
Applications
E, EIA
Kukla DA. et. al. Primary Human Hepatocytes Maintain Long-term Functions in Porous Silk Scaffolds Containing Extracellular Matrix Proteins. (2020)
Applications
E, EIA
Scharer CD et al. Antibody-secreting cell destiny emerges during the initial stages of B-cell activation. (2020)
Applications
E, EIA
Shao W et al. Serum lipoprotein–derived fatty acids regulate hypoxia-inducible factor. (2020)
Applications
E, EIA
Harshbarger W et al. Convergent structural features of respiratory syncytial virus neutralizing antibodies and plasticity of the site V epitope on prefusion F. (2020)
Applications
E, EIA
Ferdman J et al. Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccine. (2019)
Applications
E, EIA
Ciferri et al. Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies. (2015)
Applications
E, EIA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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