DNA Calf Thymus
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Datasheet

DNA Calf Thymus

MB-102-0025
5 x 5 mg
Other
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$125.00 /Per Item
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Description

Application Note

This product is a convenient, ready-to-use solution of calf thymus DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This product is also an excellent substrate for deoxyribonuclease or DNA polymerase. This solution contains sheared single stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency. Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from calf thymus DNA by mechanical shearing and heat denaturation to an average size of 100 to 2000 base pairs. To reverse any renaturation occurring during storage this material should be briefly boiled and rapidly chilled prior to use.

Purity/Specificity

Specially prepared from Calf thymus by a method to remove contaminating RNA and protein.

Storage Condition

Store vials at -20° C upon receipt. Expiration date is six (6) months from date of opening vial.

DNA (Calf Thymus) (Packaged 5 x 5 mg) - MB-102-0025
DNA Calf Thymus, ready-to-use solution of calf thymus DNA
Other
DNA
Liquid (sterile filtered)
Dry Ice
5 mg/ml by dry weight
0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
Ma Y. et al. Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine. (2020)
Applications
Other
Ali SMH et al. Copper (II) complexes of substituted salicylaldehyde dibenzyl semicarbazones: synthesis, cytotoxicity and interaction with quadruplex DNA. (2014)
Applications
Undefined
Lee WY ey al. DNA binding and nucleolytic properties of Cu(ii) complexes of salicylaldehyde semicarbazones. (2012)
Applications
Other
Chitranshi P et al. Investigation on the interactions of NiCR and NiCR-2H with DNA. (2010)
Applications
Other

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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