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10X PBS pH 7.2

MB-008 MB-008-4000
1 L 4 L
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$78.00 /Per Item
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Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.


Phosphate buffered saline, Phosphate buffered solution, PBS, 10X PBS

Application Note

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished pharmaceutical grade water (WFI).


10X PBS buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Storage Condition

Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) - MB-008
Expiration date is six (6) months from date of opening.
10X N/A
See application note.

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  • Single-cell analyses of Crohn's disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions.;2021;Jaeger N et al.;PubMed
  • Lower PDL1, PDL2, and AXL Expression on Lung Myeloid Cells Suggests Inflammatory Bias in Smoking and Chronic Obstructive Pulmonary Disease.;2020;Vasudevan S et al.;PubMed
  • Human immune system variation during 1 year.;2020;Lakshmikanth T et al.;PubMed
  • High-parameter immune profiling with CyTOF.;2020;Sahaf B et al.;PubMed
  • Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy.;2020;Colomb F et al.;PubMed
  • Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19;2020;Rodriguez L et al.;PubMed
  • SARS-CoV-2-specific T cells exhibit phenotypic features of helper function, lack of terminal differentiation, and high proliferation potential.;2020;Neidleman J et al.;PubMed
  • Profiling of the Human Natural Killer Cell Receptor-Ligand Repertoire.;2020;Vendrame E et al.;PubMed
  • Immune profiling of human gut-associated lymphoid tissue identifies a role for isolated lymphoid follicles in priming of region-specific immunity.;2020;Fenton TM et al.;PubMed
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  • Gene regulatory programs conferring phenotypic identities to human NK cells.;2019;Collins PL et al.;PubMed
  • Stereotypic immune system development in newborn children.;2018;Olin A et al.;PubMed
  • Comparison of CyTOF assays across sites: Results of a six-center pilot study.;2018;Leipold MD et al.;PubMed
  • Cytof measurement of immunocompetence across major immune cell types.;2017;Subrahmanyam PB et al.;PubMed
  • Phenotyping of live human PBMC using CyTOFTM mass cytometry.;2015;Leipold MD et al.;PubMed
  • Intracellular cytokine staining on PBMCs Using CyTOF™ mass cytometry.;2015;Lin D et al.;PubMed
  • Cytokine-stimulated phosphoflow of PBMC using CyTOF mass cytometry.;2015;Fernandez R et al.;PubMed