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Blotto A Pre-Mixed

500 mL
$48.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below).  These products are intended to block non-specific binding of proteins in various immunological assays.  Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to and during incubation with primary antibody.

Product Details

Blotto A Pre-Mixed - for a 1X Solution of Immunoanalytical Grade 5% Non-Fat Dry Milk in TBS with Tween-20 - B552-0500
Blotto A Pre-Mixed, TBS, 5% non-fat dry milk, 0.05% TWEEN-20, blocking reagents for western or ELISA

Target Details

This product contains highly purified immunoanalytical grade non-fat dry milk dissolved in a solution of Tris buffered saline.

Application Details

Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.  BLOTTO A is a convenient general purpose blocking agent.  BLOTTO B is recommended for blocking when phospho specific antibodies are used.  BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025).  Users should consider the addition of Tween-20, protease inhibitors and phosphatase inhibitors when required.  Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.  We recommend using 1X working solutions of BLOTTO A or BLOTTO B directly for blocking purposes and primary antibody dilution and incubation.


Restore with deionized water (or equivalent)

Shipping & Handling

Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Working solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen. Contains TBS and 5% non-fat dry milk. Adding the entire contents of the attached vial containing 25% TWEEN-20 (using a transfer pipette) to 500 ml of BLOTTO A results in a final concentration of 0.05% TWEEN-20. Mix well prior to use. The resulting buffer is a 1X working solution.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.