Blocking Buffer Sampler Kit

Description

Background

Rockland lyophilized NORMAL GOAT SERUM (NGS) is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications, BOVINE SERUM ALBUMIN is suitable dilute solutions of antibody, use as a blocking agent to reduce non-specific binding, 2X Blocking Buffer for Fluorescent Western Blotting is optimized for Fluorescent Western blotting and other immunoassays. ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free), 10X TBS Fish Gel Concentrate (Azide and Mercury free).

Application Note

Blocking Buffer Sampler Kit contains: NORMAL GOAT SERUM (NGS), BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free), Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - Designed for ELISA assays, 10X TBS Fish Gel Concentrate (Azide and Mercury free) is a blocking agent for immunoassays and western blot.

Purity/Specificity

Lyophilized normal goat serum was prepared from normal goat serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. BSA has Purity (%): 100% by Agarose Zone Electrophoresis Protease: <0.005 units/mg by Enzymatic Assay. And no detectable IgG by Radial Immunodiffusion. Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell blocking buffer with stabilizer, and Fish gel concentrate were aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The products were tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and found to be negative for bacteria.