BCIP/NBT Membrane Alkaline Phosphatase Substrate
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BCIP/NBT Membrane Alkaline Phosphatase Substrate

100 mL
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$109.00 /Per Item
Availability: Ships in approximately 14 days
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BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

Application Note

BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.


pH: 9.28 +/- 0.1 QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per Immunoblot: PASS

Storage Condition

Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light. No special shipping conditions or precautions are required.

5-Bromo-4-chloro-3-indonyl phosphate, X-phosphate, XP, Nitro-blue-tetrazolium chloride, Alkaline Phosphatase Substrate for Membranes, Alkaline Phosphatase Western Blot Substrate
Liquid - clear to pale yellow
Expiration date is one (1) year from date of opening.
Proprietary buffer with enhancer

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.