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BCIP/NBT Membrane Alkaline Phosphatase Substrate

100 mL
$122.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


BCIP/NBT (5-Bromo-4-chloro-3'-indolyphosphate p-toluidine salt/Nitro blue tetrazolium chloride) is utilized in combination as a chromogenic substrate for alkaline phosphatase (AP) in various applications, including WB and IHC. The reaction produces an insoluble dark purple/blue precipitate. This substrate is particularly useful for chromogenic detection in blotting and in situ hybridization assays due to its high sensitivity and resolution.

Product Details

BCIP/NBT Membrane Alkaline Phosphatase Substrate - NBT-100
5-Bromo-4-chloro-3-indonyl phosphate, X-phosphate, XP, Nitro-blue-tetrazolium chloride, Alkaline Phosphatase Substrate for Membranes, Alkaline Phosphatase Western Blot Substrate

Target Details

pH: 9.28 +/- 0.1 QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per Immunoblot: PASS

Application Details

BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.


Liquid - clear to pale yellow
Proprietary buffer with enhancer

Shipping & Handling

Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light. No special shipping conditions or precautions are required.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.