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Background

Avidin is biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat.

Product Details

Avidin - A003-01
avidin, AVD
14.4 U/mg by biotin titration method

Target Details

Avidin was prepared from chromatographically purified avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Avidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.  No reaction was observed against anti-Streptavidin.
P02701 - UniProtKB
CAC34569.1 - NCBI Protein
396260 - Gene ID

Application Details

SDS-PAGE
Microarray  - View References
Avidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized avidin.

Formulation

10 mg/mL by dry weight
None
None
None
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Avidin is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Label-Free, Multiplex Glycan Microarray Biosensor for Influenza Virus Detection. Bioconjug. Chem.
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Microarray, Microarray chip
(). Cinobufagin-induced DNA damage response activates G2/M checkpoint and apoptosis to cause selective cytotoxicity in cancer cells. Cancer Cell International
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IF, Confocal Microscopy
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Bead Conjugation
(). Autonomous bacterial localization and gene expression based on nearby cell receptor density. Molecular Systems Biology
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Electronic Absorption and Fluorescence Spectroscopy; IF, Confocal Microscopy
(). Bioconjugation of calcium phosphosilicate composite nanoparticles for selective targeting of human breast and pancreatic cancers in vivo. Acs Nano
Applications
Bioconjugation EDC - Antibody Complex
(). Human cytomegalovirus immediate early interaction with host nuclear structures: definition of an immediate transcript environment. The Journal of Cell Biology
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.