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ABTS ELISA Peroxidase Substrate

ABTS-100
100 mL
ELISA
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$109.00 /Per Item
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Description

Background

ABTS ELISA Peroxidase Substrate (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) is a chromogenic substrate use to visualize reactivity of certain enzymes, particularly in immunosorbent assays. ABTS ELISA Peroxidase Substrate is commonly used with peroxidases, but is amenable to many other enzymes. In the presence of active enzyme, the substrate becomes a soluble green product detectable at 405nm.

Synonyms

2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ABTS ELISA Peroxidase Substrate, chromogenic substrate

Application Note

ABTS ELISA PEROXIDASE SUBSTRATE is a ready to use substrate - No dilution required. Read absorbance of blue green color at 405 nm. The color formation as a function of time can be recorded at intervals or the reaction may be stopped by addition of acid after 30 mins. A 0.625 Mol L-1 Oxalic Acid stop solution is recommended, color is stable for 1hr.

Purity/Specificity

pH: 4.0 +/- 0.05 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Storage Condition

Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

ABTS ELISA PEROXIDASE SUBSTRATE - ABTS-100
ELISA
Unconjugated
Liquid - clear to very pale green
Ambient
Expiration date is one (1) year from date of opening.
1X
Proprietary buffer with enhancer

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  • Localization of Aggregatibacter actinomycetemcomitans cytolethal distending toxin subunits during intoxication of live cells.;2012;Damek-Poprawa M et al.;PubMed
  • Evaluation of the humoral immune response to the cytolethal distending toxin of Aggregatibacter actinomycetemcomitans Y4 in subjects with localized aggressive periodontitis.;2009;Xynogala I et al.;PubMed
  • Role of Aromatic Amino Acids in Receptor Binding Activity and Subunit Assembly of the Cytolethal Distending Toxin of Aggregatibacter actinomycetemcomitans.;2008;Cao L et al.;PubMed
  • Characterization of point mutations in the cdtA gene of the cytolethal distending toxin of Actinobacillus actinomycetemcomitans.;2005;Cao L et al.;PubMed
  • Nasal secretory response to allergen provocation.;1988;Cohen RA et al.;PubMed