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Datasheet SDS

ABTS ELISA Peroxidase Substrate

5 References
ABTS-100
100 mL
Liquid - clear to very pale green
ELISA
$122.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a colorimetric substrate suitable for peroxidase (HRP) enzyme labeling, producing a green color upon oxidation that is measurable at 405-410 nm. ABTS is preferred for applications requiring a soluble end product and is compatible with both plate readers and spectrophotometers.

Product Details

ABTS ELISA Peroxidase Substrate - ABTS-100
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ABTS ELISA Peroxidase Substrate, colorimetric substrate

Target Details

pH: 4.0 +/- 0.05 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Application Details

ELISA
ABTS ELISA Peroxidase Substrate is a ready to use substrate - No dilution required. Read absorbance of blue green color at 405 nm. The color formation as a function of time can be recorded at intervals or the reaction may be stopped by addition of acid after 30 mins. A 0.625 Mol L-1 Oxalic Acid stop solution is recommended, color is stable for 1hr.

Formulation

1X
Proprietary buffer with enhancer

Shipping & Handling

Ambient
Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Expiration date is one (1) year from date of receipt.
(). Localization of Aggregatibacter actinomycetemcomitans cytolethal distending toxin subunits during intoxication of live cells. Infect Immun.
Applications
E, EIA
(). Evaluation of the humoral immune response to the cytolethal distending toxin of Aggregatibacter actinomycetemcomitans Y4 in subjects with localized aggressive periodontitis. Oral Microbiol Immunol.
Applications
E, EIA
(). Role of Aromatic Amino Acids in Receptor Binding Activity and Subunit Assembly of the Cytolethal Distending Toxin of Aggregatibacter actinomycetemcomitans. Infect Immun.
Applications
E, EIA
(). Characterization of point mutations in the cdtA gene of the cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Mol Microbiol.
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E, EIA
(). Nasal secretory response to allergen provocation. Clin Allergy.
Applications
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.