Swine IgG (H&L) Antibody Biotin Conjugated
Swine IgG (H&L) Antibody Biotin Conjugated - Western Blot
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Datasheet

Swine IgG (H&L) Antibody Biotin Conjugated

Goat Polyclonal

614-1602
2 mg
WB
Goat
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$124.00 /Per Item
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Description

Background

Anti-Swine IgG (H&L) generated in goat detects swine Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG is conjugated to Biotin.

Application Note

Anti-Swine IgG (H&L) Biotin Conjugated Antibody has been tested by western blot and assayed against 1.0 ug of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:30,000 to 1:150,000 of the reconstitution concentration is suggested for this product.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Swine IgG and Swine Serum.

Anti-SWINE IgG (H&L) (GOAT) Antibody Biotin Conjugated - 614-1602
Goat Anti Swine IgG Biotin Conjugated Antibody, Goat Anti-Swine IgG Biotin Conjugated Antibody, Goat Anti-Swine IgG Antibody Biotin Conjugation
WB
Swine
Goat
Biotin
Polyclonal
IgG
Swine IgG whole molecule
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.