Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation
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Datasheet

Mouse IgG2b (Gamma 2b chain) Antibody Peroxidase Conjugated

Rabbit Polyclonal

610-4342
1 mg
ELISA, WB
Mouse
Rabbit
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Description

Background

Anti-Mouse IgG2b peroxidase conjugated antibody generated in rabbit detects specifically mouse IgG2b. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Application Note

Anti-Mouse IgG2b peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse or human heavy or light chain isotypes.

Anti-MOUSE IgG2b (Gamma 2b chain) (RABBIT) Antibody Peroxidase Conjugated - 610-4342
Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG2b Antibody HRP Conjugation
ELISA
WB
Mouse
Mouse
Rabbit
Peroxidase (Horseradish)
Polyclonal
IgG
Native Protein
Anti-Mouse IgG2b was produced by repeated immunization with mouse IgG2b heavy chain in rabbit.
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Krieger et al. Cationic domains in particle-forming and assembly-deficient HBV core antigens capture mammalian RNA that stimulates Th1-biased antibody responses by DNA vaccination. Scientific Reports (2018)
Applications
E, EIA
Wojdyla K et al. The SNO/SOH TMT strategy for combinatorial analysis of reversible cysteine oxidations. J Proteomics. (2015)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.