Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody DyLight™ 405 Conjugated
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Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody DyLight™ 405 Conjugated

Rabbit Polyclonal

610-446-C46 610-446-C46-0.5
100 µg 5 x 100 µg
WB
Mouse
Rabbit
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$99.00 /Per Item
$359.00 /Per Item
100 µg $99.00
5 x 100 µg $359.00
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Description

Background

Anti-Mouse IgG DyLight405 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Mouse IgG subclass pan reactive Secondary Antibody is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Rabbit anti-mouse IgG antibody DyLight™ 405 is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Mouse IgG secondary antibody is available in a variety of formats.

Purity/Specificity

Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Rabbit Anti-Mouse IgG shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.

Anti-MOUSE IgG (gamma 1, 2a, 2b and 3 chain) (RABBIT) Antibody DyLight™ 405 Conjugated - 610-446-C46
rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody DyLight™ 405 conjugation, rabbit anti-Mouse IgG DyLight™405 conjugated Antibody
WB
Mouse
Rabbit
IgG (gamma 1, 2a, 2b and 3 chain)
DyLight™ 405
Polyclonal
IgG
Native Protein
highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
2.0 moles DL405 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Zu et al. Tan IIA inhibits H1299 cell viability through the MDM4‑IAP3 signaling pathway. Molecular Medicine Reports (2018)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.