Mouse IgG F(c) Antibody DyLight™ 488 Conjugated
Western blot using Goat Anti-Mouse IgG Fc DyLight™488
Anti-Histone and Anti-Tubulin Antibodies - Immunofluorescence Microscopy
DyLight™ 488 Fluorescence Spectra.
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Mouse IgG F(c) Antibody DyLight™ 488 Conjugated

Goat Polyclonal

1 References
610-141-003
100 µg
WB, Dot Blot
Mouse
Goat
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Background

Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Product Details

Goat Anti-Mouse IgG Fc Antibody DyLight™ 488 Conjugated - 610-141-003
Goat Anti Mouse IgG F(c) Antibody DyLight™ 488 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 488 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 488 Conjugated
Goat
IgG Fc
DyLight™ 488
Polyclonal
IgG

Target Details

Mouse
Mouse IgG F(c) fragment
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against Mouse IgG F(ab)  This antibody will react with heavy chains of Mouse IgG.  Minimal reactivity is expected against other Mouse immunoglobulins.

Application Details

Dot Blot
WB  - View References
Anti-Mouse IgG F(c) DyLight 488 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
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Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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