Human IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
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Datasheet

Human IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

Goat Polyclonal

609-102-123
1 mg
Dot Blot
Goat
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$212.00 /Per Item
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Description

Background

Anti-Human IgG (H&L) Fluorescein generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains — two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Human IgG (H&L) Fluorescein Conjugated Antibody has been tested by dot blot. Secondary antibodies aid in the detection or sorting of target antigens by binding to the primary antibody. Anti-Human IgG (H&L) Fluorescein antibody is ideal for Flow Cytometry, Immunofluorescence Microscopy as well as other antibody detection methods where highly cross adsorbed (Pre Adsorbed) methods are required.

Purity/Specificity

Secondary Antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.

Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins) - 609-102-123
goat anti-Human IgG fluorescein conjugated Antibody, goat anti-Human IgG Antibody FITC conjugation
Dot Blot
Human
Goat
Fluorescein (FITC)
Polyclonal
IgG
Human IgG whole molecule
2.19 moles FITC per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store Secondary Antibody Conjugate at 4° C prior to restoration.   For extended storage aliquot secondary antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.