Guinea Pig IgG F(c) Antibody
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Datasheet

Guinea Pig IgG F(c) Antibody

Goat Polyclonal

606-1103
2 mg
WB
Goat
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Description

Background

Anti-Guinea Pig IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of Guinea Pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Guinea Pig IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

Application Note

Anti-Guinea Pig IgG F(c) antibody has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-guinea Pig IgG F(c) Antibody.

Purity/Specificity

Anti-Guinea Pig IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(ab).

Anti-GUINEA PIG IgG F(c) (GOAT) Antibody - 606-1103
Guinea Pig IgG F(c) Antibody, Goat-a-Guinea Pig F(c), Guinea Pig F(c) in Goat, Goat anti-guinea pig F(c) Secondary Antibody.
WB
Guinea Pig
Goat
Polyclonal
IgG
Guinea Pig IgG F(c) fragment
Liquid (sterile filtered)
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Wet Ice
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of opening.
Takada M et al. Experimental allergic conjunctivitis in guinea pigs induced by Japanese cedar pollen. Biol Pharm Bull. (2000)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.