Goat IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed
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Datasheet

Goat IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed

Rabbit Polyclonal

605-452-013S 605-452-013
100 µg 500 µg
WB
Rabbit
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$99.00 /Per Item
$318.00 /Per Item
100 µg $99.00
500 µg $318.00
Availability: Ships on approximately 01/14/2023 Availability: Ships next business day
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Note

GOAT IgG (H&L) conjugated to ATTO 488 has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

Anti-GOAT IgG (H&L) conjugated to ATTO 488 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against Human, Mouse or Rabbit Serum Proteins.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.

Storage Condition

Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Anti-GOAT IgG (H&L) (RABBIT) Antibody ATTO 488 Conjugated (Min X Hu, Ms, Rb Serum Proteins) - 605-452-013
rabbit anti-Goat IgG ATTO 488 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO488 Conjugation
WB
Goat
Rabbit
ATTO 488
Polyclonal
IgG
3.8 moles AT488 per mole of IgG
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
500 µL

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.