F(ab')2 Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated
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Datasheet

F(ab')2 Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Goat Polyclonal

712-105-002
500 µg
ELISA
Goat
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Description

Background

F(ab')2 Anti-Rat IgG Antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. F(ab')2 Anti-Rat IgG Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

Application Note

F(ab')2 Anti-Rat IgG Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for F(ab')2 Anti-Rat IgG Antibody.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

F(ab')2 Anti-RAT IgG [H&L] (GOAT) Antibody Alkaline Phosphatase Conjugated - 712-105-002
Goat F(ab')2 Anti-Rat IgG Alkaline Phosphatase Conjugated Antibody, Goat F(ab')2 Anti-Rat IgG Antibody alk phos Conjugation
ELISA
Rat
Goat
Alkaline Phosphatase (Calf Intestine)
Polyclonal
IgG F(ab')2
Rat IgG whole molecule
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.