F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
Fab2 Mouse IgG Antibody Peroxidase Conjugated - Reducing Western Blot
Fab2 Mouse IgG Antibody Peroxidase Conjugated Pre-absorbed - Western Blot
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Datasheet

F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Goat Polyclonal

710-1332
500 µg
ELISA, WB, IHC
Mouse
Goat
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Description

Background

F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Application Note

F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated Min X Bv Hs Hu Rb Rt & Sh Serum Proteins - 710-1332
Goat F(ab')2 Anti-Mouse IgG Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgG HRP conjugated Antibody
ELISA, WB
IHC
 - View References
Mouse
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG F(ab')2
Native Protein
Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
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Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.