F(ab')2 Mouse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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Datasheet

F(ab')2 Mouse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Goat Polyclonal

710-1531
500 µg
ELISA
Mouse
Goat
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Background

F(ab')2 Anti-Mouse IgG F(ab')2 Alkaline Phosphatase Antibody generated in goat detects Mouse F(ab')2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Product Details

Goat F(ab')2 Anti-Mouse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated (Min X Bv Hs Hu Rb Rt & Sh Serum Proteins) - 710-1531
Goat F(ab')2 Anti-Mouse IgG F(ab')2 Antibody alkaline phosphatase Conjugation, Goat Fab2 Anti-Mouse IgG Fab2 alk phos Conjugated Antibody
Goat
IgG F(ab')2
Alkaline Phosphatase (AP)
Polyclonal
IgG F(ab')2

Target Details

Mouse
Mouse IgG F(ab')2 fragment
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2  and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Application Details

ELISA
F(ab')2 Anti-Mouse IgG F(ab')2 Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product.  Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Formulation

Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.09% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Shipping & Handling

Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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