F(ab')2 Human IgM Fc5u Antibody Alkaline Phosphatase Conjugated
ELISA Results of F(ab')2 Anti-Human IgM Fc5µ (Goat) Antibody Alkaline Phosphatase Conjugated
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Datasheet

F(ab')2 Human IgM Fc5u Antibody Alkaline Phosphatase Conjugated

Goat Polyclonal

709-1531
500 µg
ELISA
Human
Goat
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Description

Background

F(ab')2 Anti-Human IgM Fc5µ Alkaline Phosphatase Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5µ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Application Note

F(ab')2 Anti-Human IgM Fc5µ Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. F(ab')2 Anti-Human IgM Fc5µ antibody has been assayed against 1.0 µg of Human IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG or Human IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.

F(ab')2 Anti-HUMAN IgM Fc5µ (GOAT) Antibody Alkaline Phosphatase Conjugated - 709-1531
Goat F(ab')2 Anti-Human IgM Fc5µ Antibody Alkaline Phosphatase Conjugation, Goat Fab2 Anti-Human IgM Fc5µ alk phos Conjugated Antibody
ELISA
Human
Goat
IgM Fc5µ
Alkaline Phosphatase (Calf Intestine)
Polyclonal
IgG F(ab')2
Human IgM Fc5µ fragment
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.