F(ab')2 Dog IgG (H&L) Antibody Peroxidase Conjugated
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Datasheet

F(ab')2 Dog IgG (H&L) Antibody Peroxidase Conjugated

Rabbit Polyclonal

304-4302
20 mg
Dog
Rabbit
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$293.50 /Per Item
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Description

Background

Anti-DOG IgG Antibody is a F(ab')2 fragment and is Peroxidase Conjugated. Anti-Dog antibody detects Dog or canine IgG heavy and light chains. F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F(ab')2 Anti-Dog antibody is ideal for investigators involved in serum protein component research.

Application Note

Dog antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for anti-dog antibody.  F(ab')2 Anti-Dog IgG is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

F(ab')2 Anti-DOG IgG Antibody is Peroxidase Conjugated. The F(ab’)2  fragment of a rabbit IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum.  No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

F(ab')2 Anti-DOG IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 304-4302
Rabbit F(ab')2 anti-Dog IgG Antibody Peroxidase Conjugated, Rabbit F(ab')2 anti-God IgG HRP conjugated antibody
Dog
Rabbit
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG F(ab')2
Dog IgG whole molecule
Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
None
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.