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F(ab')2 Chicken IgG F(ab')2 Antibody Fluorescein Conjugated

Rabbit Polyclonal

303-4204
20 mg
Lyophilized
Chicken
Rabbit
$292.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

F(ab')2 Anti-Chicken IgG F(ab')2 Fluorescein Antibody generated in rabbit detects Chicken F(ab')2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Product Details

Rabbit F(ab')2 Anti-Chicken IgG F(ab')2 Antibody Fluorescein Conjugated - 303-4204
Rabbit F(ab')2 anti-Chicken IgG F(ab')2 Antibody Fluorescein Conjugated, Rabbit F(ab')2 anti-Chicken IgG Fab2 fragment FITC conjugated antibody, Rabbit F(ab')2 anti-Chicken IgY F(ab')2 Antibody Fluorescein Conjugated, Rabbit F(ab')2 anti-Chicken IgY Fab2 fragment FITC conjugated antibody
Rabbit
IgG F(ab')2
Fluorescein (FITC)
Polyclonal
IgG F(ab')2

Target Details

Chicken
Chicken IgG F(ab')2 fragment
This product is a F(ab’)2  fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab’)2  and Chicken Serum.  No reaction was observed against Chicken IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.

Application Details

F(ab')2 Anti-Chicken IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.