F(ab')2 Bovine IgG F(ab')2 Antibody Peroxidase Conjugated

Datasheet

F(ab')2 Bovine IgG F(ab')2 Antibody Peroxidase Conjugated

Name: F(ab')2 Bovine IgG F(ab')2 Antibody Peroxidase Conjugated
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 301-4304

Size: 20 mg

Reactivity: Bovine

Host Species: Rabbit

$325.00 /Per Item

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Background

F(ab')2 Anti-Bovine IgG F(ab')2 Peroxidase Antibody generated in rabbit detects Bovine F(ab')2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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Specifications for F(ab')2 Bovine IgG F(ab')2 Antibody Peroxidase Conjugated

Product Details

Description: Rabbit F(ab')2 Anti-Bovine IgG F(ab')2 Antibody Peroxidase Conjugated - 301-4304

Synonyms: rabbit F(ab')2 anti-Bovine IgG F(ab')2 peroxidase Conjugated Antibody, rabbit F(ab')2 anti-Bovine IgG F(ab')2 Antibody HRP Conjugation, rabbit Fab2 anti-Bovine IgG Fab2 Antibody HRP Conjugation

Host Species: Rabbit

Specificity: IgG F(ab')2

Conjugate: Peroxidase (HRP)

Clonality: Polyclonal

Format: IgG F(ab')2

Target Details

Reactivity: Bovine

Immunogen: Bovine IgG F(ab')2 fragment

Purity/Specificity: This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.

Application Details

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Formulation

Physical State: Lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.