Fab Goat IgG Antibody Biotin Conjugated
ELISA of Donkey Fab Anti-GOAT IgG Antibody Biotin Conjugated
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Datasheet

Fab Goat IgG Antibody Biotin Conjugated

Donkey Polyclonal

805-7602
1 mg
ELISA, IHC
Goat
Donkey
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$186.00 /Per Item
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Description

Background

Fab Anti-Goat IgG Antibody generated in donkey detects goat IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Fab Anti-Goat IgG Biotin Conjugated antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Fab Anti-Goat IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum.  No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

Fab Anti-GOAT IgG (H&L) (DONKEY) Antibody Biotin Conjugated - 805-7602
Donkey Fab Anti-Goat IgG Biotin Conjugated Antibody, Donkey Fab Fragment Anti-Goat IgG Antibody Biotin Conjugation
ELISA
IHC
Goat
Donkey
IgG (H&L)
Biotin
Polyclonal
IgG Fab
Goat IgG whole molecule
10-20 moles BAC per mole of IgG Fab
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Rowe T et al. Modeling host responses in ferrets during A/California/07/2009 influenza infection. Virology. (2010)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.