Fab Chicken IgG (H&L) Antibody Peroxidase Conjugated
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Datasheet

Fab Chicken IgG (H&L) Antibody Peroxidase Conjugated

Rabbit Polyclonal

803-4302
500 µg
WB
Chicken
Rabbit
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$226.00 /Per Item
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Description

Background

Fab Anti-Chicken IgG Peroxidase Antibody generated in rabbit detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

Application Note

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.  No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).

Fab Anti-CHICKEN IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated - 803-4302
Rabbit Fab Anti-Chicken IgG Antibody Peroxidase Conjugation, Rabbit Fab Anti-Chicken IgG HRP Conjugated Antibody, Rabbit Fab Anti-Chicken IgY Antibody Peroxidase Conjugation, Rabbit Fab Anti-Chicken IgY HRP
WB
Chicken
Rabbit
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG Fab
Chicken IgG whole molecule
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Loch CM et al. A microarray of ubiquitylated proteins for profiling deubiquitylase activity reveals the critical roles of both chain and substrate. Biochim Biophys Acta. (2012)
Applications
WB, IB, PCA

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If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.