Chicken IgM (mu chain) Antibody Biotin Conjugated
Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated
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Datasheet

Chicken IgM (mu chain) Antibody Biotin Conjugated

Goat Polyclonal

603-106-007
1 mg
ELISA, IF
Goat
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Description

Background

Anti-Chicken IgM antibody specifically detects chicken IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Application Note

Anti-Chicken IgM Biotin Conjugated Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 is suggested for this product.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Chicken IgM and Chicken Serum.  No reaction was observed against other chicken heavy or light chain proteins.

Anti-CHICKEN IgM (mu chain) (GOAT) Antibody Biotin Conjugated - 603-106-007
goat anti-Chicken IgM (mu chain) Antibody Biotin Conjugation, goat anti-Chicken IgM mu chain biotin Conjugated Antibody
ELISA
IF
Chicken
Goat
Biotin
Polyclonal
IgG
Chicken IgM whole molecule
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Wang J et al. Growth of B Cell Receptor Microclusters Is Regulated by PIP 2 and PIP 3 Equilibrium and Dock2 Recruitment and Activation. Cell Rep. (2017)
Applications
IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.