Chicken IgG (H&L) Antibody Fluorescein Conjugated
Western Blot of Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody
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Datasheet

Chicken IgG (H&L) Antibody Fluorescein Conjugated

Goat Polyclonal

603-102-002
2 mg
Dot Blot, WB, ELISA, Functional Assay
Chicken
Goat
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Description

Background

Anti-Chicken IgG Fluorescein Antibody generated in goat detects chicken IgY. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Note

Anti-Chicken IgG Fluorescein Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Chicken IgG and Chicken Serum.

Anti-CHICKEN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated - 603-102-002
goat anti-Chicken IgG Fluorescein Conjugated Antibody, goat anti-Chicken IgG Antibody FITC Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY Fluorescein Conjugated Antibody, goat anti-Chicken IgY Antibody FITC Conjugation
Dot Blot, WB
ELISA, Functional Assay
Chicken
Goat
IgG (H&L)
Fluorescein (FITC)
Polyclonal
IgG
Chicken IgG whole molecule
3.7 moles FITC per mole of IgG
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Fabian BT. et al. Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens. Parasit Vectors. (2020)
Applications
E, EIA; Other

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.