Datasheet

UBE2N (UBC13) protein-HIS Epitope

Name: UBE2N (UBC13) protein-HIS Epitope
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-W90

Size: 50 µg

Reactivity: H. sapiens (Human)

Origin: Human

Expressed in: E. coli

This product is discontinued

Background

UBE2N (UBC13) or ubiquitin-conjugating enzyme E2N is a member of the E2 ubiquitin-conjugating enzyme family which plays a role in DNA post-replication repair. UBE2N gene encodes a novel human E2 that may be involved in protein degradation in the muscles and testis (1). UBE2N plays a role in initiation of homologous recombination during double-strand break repair (2). UBE2N activity may have therapeutic uses in controlling inflammatory responses and it is also important in the induction of immune responses. UBE2N Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

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Specifications for UBE2N (UBC13) protein-HIS Epitope

Product Details

Description: UBE2N(UBC13) recombinant protein-HIS Epitope - 009-001-W90

Synonyms: UBC13, UbcH-ben, Ubiquitin-conjugating enzyme E2 N, Ubiquitin carrier protein N

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: UBE2N

Reactivity: H. sapiens (Human)

Purity/Specificity: Recombinant human UBE2N (UBC13) (2-end) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Database Links:

NCBI - NM_003348

Application Details

Application Note: UBE2N Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2N Protein is suitable for use in Western Blot. Expect a band approximately ~18kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.1 µg/µL

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.