Datasheet

UBE2I protein-HIS Epitope

Name: UBE2I protein-HIS Epitope
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-U37

Size: 50 µg

Reactivity: H. sapiens (Human)

Origin: Human

Expressed in: E. coli

This product is discontinued

Background

UBE2I or ubiquitin-conjugating enzyme E2I is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair. UBE21 also known as UBC9, plays a role DNA damage repair via interaction with WT1, which is able to impose a block in cell cycle progression in eukaryotic cells (1). Listeria and probably other pathogens, dampens the host response by decreasing the sumoylation level of proteins critical for infection by targeting UBC21, an essential enzyme of the SUMO pathway. UBC21 and the SUMO pathway are crucial for proper nuclear architecture, accurate chromosome segregation, and embryonic viability (2). UBE2I Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

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Specifications for UBE2I protein-HIS Epitope

Product Details

Description: UBE2I recombinant protein-HIS Epitope - 009-001-U37

Synonyms: C358B7.1, P18, UBC9, SUMO-conjugating enzyme UBC9, Ubiquitin-conjugating enzyme E2 I

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: UBE2I

Reactivity: H. sapiens (Human)

Purity/Specificity: Recombinant full length human UBE2I was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Database Links:

NCBI - NM_003345

Application Details

Application Note: UBE2I Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2I Protein is suitable for use in Western Blot. Expect a band approximately ~19kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.1 µg/µL

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.