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Datasheet

TET3 protein-GST fusion

009-001-U15S 009-001-U15
20 µg 50 µg
H. sapiens (Human)
Human
Sf9 cells
This product is discontinued

Background

TET3 or Tet methylcytosine deoxygenase 3 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. TET3 which is a member of the ten-eleven translocation (TET) gene family is involved in myelopoiesis, and defects in TET3 have been associated with several myeloproliferative disorders. TET plays an important role in embryonic stem cell maintenance and inner cell mass cell specification (1). TET3 play an important role in the DNA methylation process (2). TET3 Protein is ideal for investigators involved in Signaling Proteins, Methylcytosine Dioxygenase Proteins, Cancer, and Cell Cycle research.

Product Details

TET3 recombinant protein-GST fusion protein - 009-001-U15
hCG_40738, tet methylcytosine dioxygenase 3
Human
Sf9 cells
Recombinant Protein

Target Details

TET3
H. sapiens (Human)
Recombinant human TET3 (724-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

TET3 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. TET3 Protein is suitable for use in Western Blot. Expect a band approximately ~140kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Liquid (sterile filtered)
0.1 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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