rRat TNF-alpha Protein
SDS-PAGE of Rat Tumor Necrosis Factor alpha Recombinant Protein
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Datasheet

rRat TNF-alpha Protein

012-001-W31-0005 012-001-W31-0020 012-001-W31-0100 012-001-W31-1000
5 µg 20 µg 100 µg 1 mg
SDS-PAGE
Rat
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$109.00 /Per Item
$260.00 /Per Item
$552.00 /Per Item
$4,010.00 /Per Item
5 µg $109.00
20 µg $260.00
100 µg $552.00
1 mg $4,010.00
Availability: Ships in approximately 14 days Availability: Ships in approximately 14 days Availability: Ships in approximately 14 days Availability: Ships in approximately 14 days
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Description

Background

Tumor Necrosis Factor alpha (TNFα) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNFα activates signals through two receptors, TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNFα can have many functions including, to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever. Recombinant rat TNFα is a non-glycosylated protein, containing 157 amino acids, with a molecular weight of 17.3 kDa.

Application Note

Tumor Necrosis Factor alpha Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Tumor Necrosis Factor alpha in immunological assays. Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Purity/Specificity

Tumor Necrosis Factor alpha purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Rat Tumor Necrosis Factor alpha Recombinant Protein - 012-001-W31-0005
TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Tnf
SDS-PAGE
Rat
E. coli
Recombinant Protein
Lyophilized
See application note.
None
None
5µl (5-50µl)
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Expiration date is six (6) months from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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