Datasheet

RhoC protein-HIS Epitope

Name: RhoC protein-HIS Epitope
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-T22

Size: 50 µg

Physical State: Liquid (sterile filtered)

Reactivity: H. sapiens (Human)

Origin: Human

Expressed in: E. coli

This product is discontinued

Specifications for RhoC protein-HIS Epitope

Product Details

Description: RhoC recombinant protein-HIS Epitope - 009-001-T22

Synonyms: ARH9, ARHC, H9, MGC1448, MGC61427, RHOH9, Rho-related GTP-binding protein RhoC

Species of Origin: Human

Expressed in: E. coli

Type: Recombinant Protein

Target Details

Gene Name: RHOC

Reactivity: H. sapiens (Human)

Purity/Specificity: Recombinant full length human RHOC was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >75% by densitometry.

Database Links:

?term=NM_175744 - Gene ID

Application Details

Application Note: RhoC Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. RhoC Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~23kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Concentration: 0.2 µg/µL

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

Background

RhoC is member of the Rho family of small GTPases which promote reorganization of the actin cytoskeleton during cell morphogenesis and motility and regulate cell shape, attachment and motility. Active RhoC signals to its downstream effector ROCK which phosphorylates and activates LIM kinase that in turn phosphorylates cofilin inhibiting its actin-depolymerizing activity (1). Overexpression of RhoC is associated with tumor cell proliferation and metastasis while dominant-negative RhoC inhibits metastasis (2). Analysis of the phenotype of cells expressing dominant-negative RhoC indicates that RhoC is important in tumor cell invasion. RhoC Protein is ideal for investigators involved in Signaling Proteins, G-Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.