Protein G Fluorescein Conjugated
Dot Blot of Protein G Fluorescein Conjugate
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Datasheet

Protein G Fluorescein Conjugated

PG00-02
1 mg
Dot Blot
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$176.00 /Per Item
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Description

Background

Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Protein G is also coupled with reporter molecules such as a fluorescein, peroxidase, biotin and other detection reagents.

Application Note

Protein G Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays.

Purity/Specificity

Protein G Fluorescein Conjugate was prepared from chromatographically pure recombinant Protein G.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein G. No reaction was observed against anti-Protein A.

PROTEIN G Fluorescein Conjugated - PG00-02
ProG, Streptococcus G protein, Protein G FITC
Dot Blot
Fluorescein (FITC)
2.15 moles FITC per mole of
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.